Details
pUC19 Vector Applications
- Cloning
- Sequencing
- Manufacturing DNA Size Markers
Vector Identity
Identity and homogeneity of pUC19 Vector were confirmed by a series of restriction enzyme digests followed by analysis of the resulting DNA fragment pattern
Storage Buffer
10 mM Tris-HCl (pH 7.8)1 mM EDTA
Notes
- pUC19 Vector manufactured by CHIMERx is≥90% supercoiled
- pUC19 Vector is ampicillin resistant
- The multiple cloning site contains recognition sequences for the following restriction enzymes: Acc I, Acc651, Apo I, Ava I, BamH I, Ban II, BfuA I, BsoB I, BspM I, Eco53K I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac II, Sal I, Sbf I, Sma I, Sph I, TspM I, Xba I and Xma I
Storage Conditions
Store at 4°C short term (2 weeks or less)Store at -80°C long term (longer than 2 weeks)Avoid multiple freeze-thaw cycles
Downloads
Certificate of Analysis -Current Lot
Categories
Specialty Reagents