Details

Description

• Removes 5´ phosphates from DNA, RNA, rNTPs and dNTPs.
• Heat inactivation in 5 minutes at 70°C.
• Resistant to chemical changes and active over a broad range of buffer conditions.
• Degradation of dNTPs prior to sequencing of PCR product.
• Can be used to remove 5-phosphates from DNA or RNA prior to 5-end labeling
• Removes 5-phosphates from linearized vector molecules to prevent selfligation of the vector during cloning procedures

Applications

• Ideal fordiagnostic immunoassays
• Immunodetection of proteins and nucleic acids following blotting experiments
• Protein dephosphorylation.

Unit Definition

One unit is the amount of enzyme required to dephosphorylate 1 µg of linearized pUC19 vector DNA in 30 min at 37°C.

Reaction Conditions

1-5 µg of DNA cut with any restriction enzyme5 µl 10 x Polar-BAP Reaction Buffer 1 µl of Polar-BAP 5 U/μl @ 50 µl H2O, nuclease free

Storage Buffer

10 mM Tris-HCl (pH 7.6 at 22°C)1 mM MgCl₂0.01 mM ZnCl₂1 mM DTT50% glycerol.

1x Polar-BAP Reaction Buffer

50 mM Bis-Tris-Propane-HCl pH 6 @ 25°C1 mM MgCl₂0.1 mM ZnCl₂

Quality Control

All preparations are assayed for contaminating endonuclease and nonspecific RNase and single- and double-stranded DNase activities.

Storage Conditions

Store at -20ºCProduct shipped on dry ice