Details

Spe I Source

Sphaerotilus species

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1 µg of Adenovirus 2 DNA in 1 hour in a total reaction volume of 50 µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

10 minutes at 65°C

Assay Unit Substrate

Ad-2 DNA

Reaction Buffer

CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1:100%Buffer 2:100%Buffer 3: 50%Buffer 4: 75%Buffer 5: 100%*Star activity

Storage Buffer

50 mM Tris-HCl (pH 7.5 at 4°C)200 mM NaCl0.1 mM EDTA0.2% (v/v) Triton 
X-100™1 mM dithiothreitol500 µg/ml bovine serum albumin50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin1µg Ad-2 DNA

Storage Conditions

Store at -20°CShipped on dry ice

Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases