Details

Pvu II Source

Proteus vulgaris

Reagents Supplied

CMX Buffer 2

Unit Definition

One unit is the amount of enzyme required to completely digest 1µg of lambda DNA in 1 hour in a total reaction volume of 50µl

Star Activity

Yes

Incubation Temperature

37°C

Heat Inactivation

No

Assay Unit Substrate

Lambda DNA

Reaction Buffer

CMX Buffer 2:10 mM Tris-HCl (pH 7.5 at 37°C)10 mM MgCl₂50 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin

Activity in Buffers

Buffer 1:75%Buffer 2:100%Buffer 3: 75%Buffer 4: 75%Buffer 5: 75%*Star activity

Storage Buffer

50 mM Tris-HCl (pH 7.4 at 4°C)50 mM KCl0.1 mM EDTA10 mM BME500 µg/ml bovine serum albumin0.1% Tween 20™50% (v/v) glycerol

Assay Conditions

10 mM Tris-HCl (pH 7.5 at 37°C)50 mM NaCl10 mM MgCl₂1 mM dithiothrietol100 µg/ml bovine serum albumin1 µg lambda DNAIncubation is at 37°C for 1 hour in a total reaction volume of 50µl

Note

To avoid star activity it is not recommended to use more than 15 units per reaction or to incubate over 1 hour

Storage Conditions

Store at -20°CShipped on dry ice

Pvu II Downloads

Certificate of Analysis PDF-Current LotMSDS PDF-Current Lot

Categories

Restriction Endonucleases