Details

Source

Thermus aquatieus

Applicatons

• Hydrolyzes pyrophosphate, a common product of biosynthetic reacions (1)
• Maintains the forward direction of reactions generating pyrophosphate
• Improves PCR amplification of problematic of long templates
• Used as an enzyme label of ELISA (2)

Unit Definition

One unit will liberate 1.0 nmol of inorganic orthophosphate per minute at 65°C (pH 7.5)

Storage Buffer

20 mM Tris-HCl (pH 8.0 at 4°C)50 mM imidazole100 mM KCl0.1 mM EDTA50% (v/v) glycerol0.5% Triton X-100

Assay Conditions

40 mM Tris-HCl (pH 7.5 at 22°C)40 mM sodium acetate40 mM imidazole1.0 mM sodium pyrophosphate.2.5 mM MgCl₂Reaction volume 100μl

Quality Control

All preparations are assayed for contaminating endonuclease, exonuclease and nonspecific RNase and single- and double-stranded DNase activites.

Storage Conditions

Store at -80°CShipped on Dry Ice

Downloads

MSDS PDF-Current Lot

References

Baykov, A.A., Kasho, V.N. and Avaeva, S.M. (1988) Anal. Biochem. 171, 271-276.2. Jerhoeven, J.A. (1986) J. Bacteriol. 168, 318-321Richter, O.M. and Schafer, G. (1992) Eur. J. Biochem. 209, 343-349