Details

Source

Escherichia coli

Applications

• Adds poly A tails to the 3’-hydroxyl terminus of RNA (1)
• Labels 3’-ends of RNA with a [α-³²P] cordycepin triphosphate (2)
• Ideal for determining molar concentration of polyadenylated RNA (3)

Unit Definition

One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid insoluble form for 10 minutes at 37°Cunder the stated assay conditions

Concentration

1 Unit/µl

Storage Buffer

25 mM Tris-HCl (pH 7.9)0.1 mM DTT0.01% (v/v) Triton X-100™1.0 mM EDTA0.5 M NaCl50% (v/v) glycerol

Assay Conditions

50 mM Tris-HCl (pH 7.9)10 mM MgCl₂2.5 mM MnCl₂250 mM NaCl25 µg tRNA0.1 mM [α-³²P] ATP50 μg bovine serum albuminReaction volume of 100 µl

Storage Conditions

Store at -20°CProduct shipped on dry ice

Downloads

Certificate of AnalysisPDF-Current LotMSDS PDF

References

(1) Sippel, A.E. (1973) Eur. J. Biochem. 37, 31-40(2) Beltz, W.R. and Ashton, S.H. (1982) Fed. Proc. 41, 1450, Abstract 68996(3) Krug, M.S., and Berger, S.L. (1986) Anal. Biochem. 153, 315-323