Details
Source
Escherichia coli RY 13
Reagents Supplied
CMX Buffer 3
Unit Definition
One unit is the amount of enzyme required to completely digest 1 µg of lambda DNA in 1 hour in a total reaction volume of 50 µl
Star Activity
Yes
Incubation Temperature
37°C
Heat Inactivation
65°C for 10 minutes
Assay Unit Substrate
Lambda DNA
Reaction Buffer
CMX Buffer 3:50 mM Tris-HCl (pH 7.5 at 37°C) 10 mM MgCl2100 mM NaCl1 mM dithiothreitol100 µg/ml bovine serum albumin
Activity in Buffers
Buffer 1: 50%Buffer 2: 100%Buffer 3: 100%Buffer 4: 75%Buffer 5: 75%*Star activity
Storage Buffer
50 mM Tris-HCl (pH 7.2 at 4°C) 300 mM NaCl0.5 mM EDTA5 mM EGTA0.2% (v/v) Triton X-100™5 mM BME5µg/ml PLL500 µg/ml bovine serum albumin50% (v/v) glycerol
Assay Conditions
See Certificate of Analysis Below
Storage Conditions
Store at -20°CShipped on dry ice
Downloads
Certificate of AnalysisLot 2404008 PDFLot 2307006 PDF
MSDSMSDS PDF
