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主营:核酸提取纯化试剂、DNA聚合酶、核酸纯化试剂盒、重组酶、DNA标记等
℡ 4000-520-616
℡ 4000-520-616
Chimerx/Rsr II/2342/500 u
产品编号:2342-01
市  场 价:¥1360.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$68.00
品      牌: Chimerx
公      司:Chimerx,Inc.
公司分类:
Chimerx/Rsr II/2342/500 u
商品介绍
Details
RsrIISource

Rhodopseudomonassphaeroides

ReagentsSupplied

CMXBuffer4

UnitDefinition

Oneunitistheamountofenzymerequiredtodigest1µgoflamBDaDNAwith≥95%efficiencyin1hourinatotalreactionvolumeof50μl

StarActivity

No

IncubationTemperature

37°C

HeatInactivation

10minutesat65°C

AssayUnitSubstrate

LambdaDNA

ReactionBuffer

CMXBuffer4:
20mMTris-Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1mMdithiothreitol
100µg/mlbovineserumalbumin

ActivityinBuffers

Buffer1:50%
Buffer2: 75%
Buffer3:25%
Buffer4:100%
Buffer5:50%

StorageBuffer

10mMTris‑HCl(pH7.5at4ºC) 
0.1mMEDTA
50mMKCl
1mMdithiothreitol
200µg/mlbovineserumalbumin
50%(v/v)glycerol

AssayConditions

20mMTris‑Acetate(pH7.5at37ºC)
50mMPotassiumAcetate
10mMMagnesiumAcetate
1.0mMdithiothreitol
100µg/mlbovineserumalbumin
1µglambdaDNA
Incubationisat37°Cfor1hourinatotalreactionvolumeof50µl

Notes
  1. LambdaDNAcontains3slow-cleavagesitesthatdonotcompletelydigestafter1hourincubation.
  2. Completedigestionofthesesitesisachievedby4UnitsofRsrIIwith1μgoflambdaDNAincubatedat37°Cfor16hours.
StorageConditions

Storeat-20°C
Shippedondryice 

Downloads

CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot

Categories

RestrictionEndonucleases

品牌介绍

Chimerx


自1992年以来,Chimerx一直在生产最优质的生物化学物质、试剂和核酸提取和纯化试剂盒,可供生命科学界使用。我们不断扩大的产品线使发现的成功率最高。我们目前提供200多个产品,还有更多的产品正在开发中,以使我们的客户处于领先地位。


Founded in Madison, WI, Chimerx has earned the reputation as a leading manufacturer of the highest quality reagents available to investigators in various fields of biotechnology research. Since 1992, Chimerx has been manufacturing the highest quality biochemicals, reagents, and nucleic acid extraction and purification kits available to the life science community. Our ever expanding product line enables the highest rate of success in discovery. We currently offer more than two hundred products, with many more currently in development to keep our customers at the cutting edge.


品牌

货号

名称

规格

Chimerx

1025

T4 Gene 32 Protein

1

Chimerx

1065

E. coli DNA Ligase

1

Chimerx

1140

Exonuclease III, E. coli

1


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