ChlorellavirusIL-3A
Note: Purifiedfromarecombinantsource(PatentNo.US005472872A)
10XCviJI*ReactionBuffer
DMSO
37°C
65°Cfor10minutes
pBR322
10XCviJI*ReactionBuffer:
20mMglycyl-glycineKOH(pH8.5)
10mMmagnesiumacetate
50mMpotassiumacetate
0.1mMATP
0.1mMdithiothreitol
Note:100% DMSOsuppliedseparately
20mMTris-acetate(pH8.0at4°C)
0.5mMEDTA
0.1mMdithiothreitol
5mMmagnesiumchloride
50mMpotassiumacetate
50%(v/v)glycerol
20mMglycylglycine-KOH(pH8.5)
10mMmagnesiumacetate
0.1mMdithiothreitol
50mMpotassiumacetate
0.1mMATP
30%DMSO
1µgpBR322
Incubationfor3hoursat37°Cinatotalreactionvolumeof25µl
Storeat-80°C
Shippedondryice
(1)CviJI*restrictionendonucleaseisinhibitedbyglycerolconcentrationsinexcessof2.5%.ThereforetheextensionofthedigestiontimeisrecommendedratherthanusingadditionalunitsofCviJI*.Alternatively,DNAsamplecanbeethanolprecipitatedandre-digested.
(2)DuetoextremefrequencyofCviJI*/CviJIrecognitionsites,stericalinterferenceofcloselylocatedrecognitionsitesisobserved.Itresultsinslowerdigestionofsuchsites.Inconsequence,thegeneratedoligonucleotidefragmentsarerarelyshorterthan15bpthatmakesthemidealforanonymousprimerapplications.
(3)CviJI*reactionbuffercontainsDMSO,whichdoesnotinterferewithfurtherenzymaticmanipulations(ligations,labeling,etc).Ifthesampleisintendedforelectrophoresis,ethanolprecipitatationofthereactionmixtureaftercompleteddigestionisstronglyrecommendedinordertoavoiddiffusedbandsonagaroseorpolyacrylamidegels.
CertificateofAnalysisPDF -CurrentLot
MSDSPDF -CurrentLot
(1)Xia,Y.,Burbank,D.,Uher,L.,Rabussay,D.andVanEtten,J.NucleicAcidsRes.15,6075-6090
(2)Fitzgerald,M.C.,Skowron,P.,VanEtten,J.L.,Smith,L.M.andMead,D.A.(1992)NucleicAcidsRes.20,3753-3762
(3)Skowron,P.M,Swaminathan,N.,McMaster,K.,George,D.,VanEtten,J.andMead,D.Gene157(1995)37-41
(4)Mead,D.,Swaminathan,N.,VanEttenJ.andSkowron,P.M.:RecombinantCviJIrestrictionendonuclease.(1995)UnitesStatesPatentnoUS005472872A
(5)Swaminathan,N.,McMaster,K.,Skowron,P.andMead,D.A.AnalyticalBiochemistry255(1998)133-141
Chimerx
自1992年以来,Chimerx一直在生产最优质的生物化学物质、试剂和核酸提取和纯化试剂盒,可供生命科学界使用。我们不断扩大的产品线使发现的成功率最高。我们目前提供200多个产品,还有更多的产品正在开发中,以使我们的客户处于领先地位。
Founded in Madison, WI, Chimerx has earned the reputation as a leading manufacturer of the highest quality reagents available to investigators in various fields of biotechnology research. Since 1992, Chimerx has been manufacturing the highest quality biochemicals, reagents, and nucleic acid extraction and purification kits available to the life science community. Our ever expanding product line enables the highest rate of success in discovery. We currently offer more than two hundred products, with many more currently in development to keep our customers at the cutting edge.
品牌 | 货号 | 名称 | 规格 |
Chimerx | 1025 | T4 Gene 32 Protein | 1 |
Chimerx | 1065 | E. coli DNA Ligase | 1 |
Chimerx | 1140 | Exonuclease III, E. coli | 1 |