Chlorella virus IL-3A
Note 1: Purified from E coli strain that carries the cloned cvijRI gene from Chlorella virus IL-3A (Patent No. US005472872A)
10X CviJ I Reaction Buffer
DMSO
One unit is the amount of enzyme required to completely digest 1 µg of pBR322 DNA in 1 hour in a total reaction volume of 25 µl
No
37°C
65°C for 10 minutes
pBR322
10X CviJ I Reaction Buffer:
20 mM glycylglycine-KOH (pH 8.5)
10 mM magnesium acetate
50 mM potassium acetate
0.1 mM dithiothreitol
Note 2: 100% DMSO supplied separately
20 mM glycyl-glycine-KOH (pH 8.5)
50 mM potassium acetate
10 mM magnesium acetate
0.1 mM dithiothreitol
10% DMSO
1 µg pBR322
Incubation for 1 hour at 37°C in a reaction volume of 25 µl
20 mM Tris-acetate (pH 8.0 at 4°C)
50 mM potassium acetate
5 mM MgCl2
0.5 mM EDTA
1 mM dithiothreitol
50% (v/v) glycerol
Store at -80°C
Shipped on dry ice
Cerftificate of Analysis PDF - Current Lot
(1) Xia,Y., Burbank, D., Uher, L., Rabussay, D. and Van Etten, J. Nucleic Acids Res.15, 6075-6090
(2) Fitzgerald,M.C., Skowron, P., Van Etten, J.L., Smith, L.M. and Mead, D.A. (1992) Nucleic Acids Res. 20, 3753-3762
(3) Mead, D., Swaminathan, N., Van Etten J. and Skowron, P.M.: Recombinant CviJI restriction endonuclease. (1995) Unites States Patent no US005472872A
(4) Skowron, P.M, Swaminathan, N., McMaster, K., George, D., Van Etten, J. and Mead, D. Gene 157 (1995) 37-41
(5) Swaminathan, N., McMaster, K., Skowron, P. and Mead, D.A. Analytical Biochemistry 255 (1998) 133-141
Chimerx
自1992年以来,Chimerx一直在生产最优质的生物化学物质、试剂和核酸提取和纯化试剂盒,可供生命科学界使用。我们不断扩大的产品线使发现的成功率最高。我们目前提供200多个产品,还有更多的产品正在开发中,以使我们的客户处于领先地位。
Founded in Madison, WI, Chimerx has earned the reputation as a leading manufacturer of the highest quality reagents available to investigators in various fields of biotechnology research. Since 1992, Chimerx has been manufacturing the highest quality biochemicals, reagents, and nucleic acid extraction and purification kits available to the life science community. Our ever expanding product line enables the highest rate of success in discovery. We currently offer more than two hundred products, with many more currently in development to keep our customers at the cutting edge.
品牌 | 货号 | 名称 | 规格 |
Chimerx | 1025 | T4 Gene 32 Protein | 1 |
Chimerx | 1065 | E. coli DNA Ligase | 1 |
Chimerx | 1140 | Exonuclease III, E. coli | 1 |